SPARC anticorps

N° du produit ABIN602083

Cet anticorps Rat Monoclonal détecte spécifiquement SPARC dans IHC, ELISA, FACS et IHC (fro). Il présente une réactivité envers Souris.

Aperçu rapide pour SPARC anticorps (ABIN602083)

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp SPARC est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-SPARC anticorps

Specificité: Recognizes mouse Osteonectin. Displays no cross-reactivity with recombinant human Osteonectin.

Purification: Protein G purified

Immunogène: Recombinant mouse Osteonectin.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-Fr (25 μg/mL)

Usage: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. ELISA: 0.5-1 μg/mL). Immunohistochemistry (frozen): 25 μg/mL detects SPARC on mouse ovary tissue. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 25 μg/mL and add 10 μLof this solution to 1-5x10^5 cells in a total reaction volume 200uL. Following a 30 minute incubation, cells should be washed with 0.1 % saponin prior to adding 10 mL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Stock: -20 °C

Stockage commentaire: Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C. Further dilutions can be made in buffer.

Détails sur SPARC

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: Osteonectin / SPARC

Sujet: Name/Gene ID: SPARC

Synonyms: SPARC, Basement-membrane protein 40, BM-40, Cysteine-rich protein, ON, Osteonectin

ID gène: 6678

UniProt: P09486

Pathways: Autophagy